The 13C-urea breath test to detect Helicobacter pylori infection: a validated simple methodology with 50 mg 13C-urea.
نویسندگان
چکیده
BACKGROUND Several modifications of the 13C-urea breath test have been published. For reasons of cost efficiency and practicability, the urea dose and measurement duration should be reduced while still maintaining excellent diagnostic accuracy. AIMS To establish a validated simple protocol for the urea breath test with 50 mg 13C-urea and to compare this protocol with the conventional urea breath test with 100 mg 13C-urea. METHODS Conventional urea breath test with 100 mg 13C-urea was performed on 152 dyspeptic patients. Full-cream cow's milk was used as the test meal. Breath tests were repeated using 50 mg 13C-urea and the breath samples were collected at baseline and at 10 (protocol t10), 15 (protocol t15) and 30 min (protocol t30). Helicobacter pylori status was assessed by rapid urease test, histology and conventional urea breath test with 100 mg 13C-urea. RESULTS With protocol t15, the best combination of sensitivity (99.1%), specificity (97.3%) and accuracy (98.7%) was obtained with a cut-off of 2.5 per thousand. There was an extremely high correlation coefficient between the three protocols and conventional 13C-urea breath test (all P < 0.001). CONCLUSIONS A urea breath test with 50 mg 13C-urea using a simple test meal and a 15-min sampling interval with a low cut-off seems to be cost-effective and convenient. In a well-standardized laboratory, this modification is not associated with any loss of diagnostic accuracy.
منابع مشابه
Low dose capsule based 13c-urea breath test compared with the conventional 13c-urea breath test and invasive tests.
CONTEXT One of the limitations of 13C-urea breath test for Helicobacter pylori infection diagnosis in Brazil is the substrate acquisition in capsule presentation. OBJECTIVES The purpose of this study was to evaluate a capsule-based 13C-urea, manipulated by the Pharmacy Division, for the clinical practice. METHODS Fifty patients underwent the conventional and the capsule breath test. Samples...
متن کامل[Validation of a simplified 13C-urea breath test method for the diagnosis of Helicobacter pylori infection].
OBJECTIVE To validate a simplified 13C-urea breath test (13C-UBT) method for the diagnosis of H. pylori infection. MATERIAL AND METHODS Patients referred for gastrointestinal endoscopy and biopsy were included, and a 13C-UBT was performed after a 6-hour fast. Breath samples were collected in 10 ml glass tubes before and 30 min after the simultaneous administration of 50 mg of 13C-urea and 2 g...
متن کاملEvaluation of the Ez-HBT Helicobacter blood test to establish Helicobacter pylori eradication.
BACKGROUND The urea blood test (Ez-HBT) has been shown to compare favourably with the urea breath test in the diagnosis of active Helicobacter pylori infection. AIM To examine the performance characteristics of the Ez-HBT Helicobacter blood test in establishing success or failure of therapy in H. pylori-infected adults using the 13C urea breath test as the reference method. METHODS 13C urea...
متن کامل13C-Urea breath test threshold calculation and evaluation for the detection of Helicobacter pylori infection in children
BACKGROUND The 13C-urea breath test (UBT) is performed in adults and children with epigastric pain for non-invasively diagnosing a suspected H. pylori infection. Criteria for UBT interpretation have not been generally agreed on and test reliability has not been established in children of different ages. This study aimed at identifying reliable UBT thresholds in children by using 251 UBTs in con...
متن کاملModified Helicobacter test using a new test meal and a 13C-urea breath test in Helicobacter pylori positive and negative dyspepsia patients on proton pump inhibitors
AIM To determine the sensitivity and specificity of the 13C-urea breath test (UBT) in patients taking proton pump inhibitors (PPIs), using a new test meal Refex. METHODS One hundred and fourteen consecutive patients with dyspepsia, 53 Helicobacter pylori (H. pylori) positive, 49 H. pylori negative, were included in the study. The patients were then given esomeprazole 40 mg for 29 consecutive ...
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ورودعنوان ژورنال:
- Alimentary pharmacology & therapeutics
دوره 16 4 شماره
صفحات -
تاریخ انتشار 2002